Egg prep
This egg prep protocol is modified from Wormbook.
- Prepare the working solution, Mix 2N NaOH and bleach, 1:1.
- Wash plate with M9, mix the M9 with the working solution, 1:1.
- Stand the tube for 5 min, shake it violently.
- Centrifugate the tube with 3000 rpm (soft braking).
- Wash the pellet with M9, three times.
- Resuspend the pellet with about 100 ul M9, and seed the eggs onto new plate.
- For a 90 mm plate, usually need 2 ml M9 to wash. At this time, you will need 2 ml working solution at first.